Triple Negative Breast Cancer (TNBC), indicated by the absence of estrogen, progesterone and human epidermal growth factor receptor 2 (HER2), is the most aggressive form of breast cancer characterized by high rates of metastasis and low survival. Among those diagnosed with TNBC, 34% contain Inhibitor of Growth 4 (ING4) deletion that is associated with poor patient outcomes. We previously showed that ING4 negatively regulates NF-B in breast cancer. Previous studies show parthenolide, a compound found in feverfew (Tanacetum parthenium) to inhibit NF-B in cervical and gastric cancer. We hypothesized that parthenolide inhibits cytokine-induced activation of NF-B in ING4 deficient TNBC cells. To test the hypothesis, previously established vectors, v2, ING4 wildtype and v2h1, ING4-deleted were synthesized in MDA-MB 231, a TNBC cell line, using a CRISPR/Cas9 system. Inflammatory cytokines, IL-1 and TNF, were tested in ING4 wildtype or ING4 deleted cells for elicited phosphorylation of NF-B, proliferation, and migration in the presence or absence of parthenolide. The results showed that TNF or IL-1 induced translocation phosphorylation of NF-B regardless of ING4 deletion. ING4 inhibited proinflammatory cytokine induced pp65, consistent with previous studies demonstrating the negative regulation of NF-B in ING4-sufficent cell lines. We found the optimal working dose of parthenolide, 100nM, had no effect on cell proliferation in the presence or absence of IL-1. Parthenolide inhibited IL-1induced phosphorylation of NF-B regardless of ING4 deletion. Parthenolide inhibited TNF-induced phosphorylation of NF-B in ING4-deleted cell lines. Moreover, parthenolide induced migration of TNBC cells regardless of ING4 presence of absence. TNF and parthenolide treated samples in ING4-deleted cell lines were found to inhibit cell migration to basal level. These results demonstrate the difference in inhibitory mechanism of parthenolide in induced phosphorylation of NF-B through proinflammatory cytokines TNF or IL-1This is demonstrated by the exclusivity of parthenolide inhibition of TNF induced phosphorylation of NF-B in ING4-deleted TNBC cell line. In contrast, parthenolide inhibition of IL-1 induced phosphorylation of NF-B occurred regardless of ING4 deletion. These results may inhibit parthenolide as an alternative to those with ING4-deleted TNBC due to its role in inducing cancer phenotype cell migration.

This thesis utilizes GIS mapping to analyze the severity of four threats: ocean acidification, sea surface temperature, artisanal fishing, and destructive fishing, in conjunction with coral species distribution. This project produced maps that depicts each of these threats and shows the distribution of its severity. Compiling this data we can see that ocean acidification is the most pressing threat in the Caribbean to coral and that neither type of fishing really has a large effect. A species named Madracis carmabi is also flagged to be of particular concern as it is severely threatened by both ocean acidification and sea surface temperature.

The purpose of this study was to determine the feasibility of a mindfulness-based intervention among pregnant women (12-20 weeks’ gestation) using a mobile meditation app, Calm. This study involved 100 participants who were recruited nationally due to the COVID-19 pandemic. This study was reviewed and approved by the Institutional Review Board of Arizona State University (STUDY STUDY00010467). All participants were provided an informed consent document and provided electronic consent prior to enrollment and participation in this study. This study was a randomized, controlled trial (trial registration: ClinicalTrials.gov NCT04264910). Participants randomized to the intervention group were asked to participate in a minimum of 10 minutes of daily meditation using a mindfulness meditation mobile app (i.e., Calm) for the duration of their pregnancy. Participants randomized to the standard of care control group were given access to the app after they gave birth. Both the intervention and control groups were administered surveys that measured feasibility outcomes, perceived stress, mindfulness, self-compassion, impact from COVID-19, pregnancy-related anxiety, depression, emotional regulation, sleep, and childbirth experience at four time points: baseline (12-20 weeks gestation), midline (24 weeks gestation), postintervention (36 weeks gestation), and follow-up survey (3-5 weeks postpartum). Data is currently being analyzed for publication.

Not only is Tyrosine one of the 20 amino acids that make proteins, but its catabolism also has many branches including a pathway that can be found in humans. Any mutations in the enzymes of this pathway can cause many disorders in humans including hereditary tyrosinemia type I. For this reason, understanding how tyrosine gets degraded in humans can help in developing therapies against disorders of the human tyrosine catabolism pathway. In this work, we explored what type of enzymes do microbes that reside within humans (the human microbiome) have to degrade tyrosine and how we can take advantage of the enzymes of the human microbiome for the betterment of human health and physiology.

Throughout the year I had the opportunity to work on a business venture that utilized dendrites as a unique identifier. Dendrites themselves are completely unique, random, branching structures that occur everywhere in nature. This creative project was inspired by the shape of the dendrite and I created a series of 12 paintings reflecting on my own unique college journey.

Aboveground-belowground relationships between vegetation and its associated soil biotic community play an important role in every terrestrial ecosystem for nutrient cycling and soil health maintenance. Deserts are especially sensitive to change and little is known about Sonoran Desert soil microbiota, while exotic herbaceous species are increasingly invading into the ecosystem with other harmful effects. In many other environments, soil communities have been associated with both plant species and plant functional type. The soil community food web depends on the sustenance brought by vegetation, and different soil community members are adapted to different diets. In this paper, we hypothesized that invasive plants would cause belowground soil communities to have greater abundance and lesser diversity than those under native, more locally established plants. To test this hypothesis, we selected four desert understory plant taxa: one native grass, one native forb, one invasive grass, and one invasive forb. We predicted that the invasive plants would be associated with a greater count of microarthropods per unit mass of soil but lesser microarthropod species diversity. The invasive plants were not statistically associated with a greater count of microarthropods per kilogram of soil nor lesser microarthropod species diversity. There was not a significant difference in abundance in the microarthropod categories between native and invasive plants, so the hypothesis was rejected. However, the invasive Erodium cicutarium was found to harbor high soil mite abundance, which warrants further study, and it is yet to be seen whether soil moisture and proximity to trees played a role in the data. The results of this study should help in generating more informed hypotheses regarding desert aboveground-belowground relationships.