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The State of Black Arizona: Volume 2

Description

The African-American community has played a historically significant role in the advancement of Arizona and our region. The future success of our state relies on our ability to strengthen our communities and empower them to meet and exceed their vast potential. This project between the community and the University was…

The African-American community has played a historically significant role in the advancement of Arizona and our region. The future success of our state relies on our ability to strengthen our communities and empower them to meet and exceed their vast potential. This project between the community and the University was undertaken to help advance a better understanding of the changing dynamics of Arizona’s African-American population and the critical issues that require our collective attention in terms of education, health care, the economy, culture and leadership.

ContributorsArizona State University (Author) / Arizona Community Foundation (Author) / Tucson Urban League (Author) / Glasper, Rufus (Author) / Ramakrishna, Pushpa (Author)

Created2009

Research and Development of a Blackboard Orientation Course for Face-to-Face Students Taking Online Courses at Washington State University-Everett

Description

Washington State University Everett could benefit from a Blackboard® online orientation course prior to their first credited online course. Research included results from student satisfaction surveys and focus groups. It was determined through both quantitative and qualitative data that students who opt into an online orientation course have the potential…

Washington State University Everett could benefit from a Blackboard® online orientation course prior to their first credited online course. Research included results from student satisfaction surveys and focus groups. It was determined through both quantitative and qualitative data that students who opt into an online orientation course have the potential for increased satisfaction and success with online coursework throughout their degree-completion experience. Once this determination was made, a fully-functioning Blackboard® orientation course was designed and developed. The course has been tested by faculty and is ready for Fall 2017 deployment as a voluntary online orientation for any student already admitted to WSU Everett.

ContributorsWilder, Corrie (Author) / Brumberger, Eva (Degree committee member) / D'Angelo, Barbara J. (Degree committee member) / Arizona State University (Publisher)

Created2017-03-20

Creating a Radio Sound Board Operation Guide for Novices

Description

The field of radio broadcast requires the cohesion of several different skill sets in order to be a success. KHEA Radio has used a traditional form of teaching, which means taking a one-on-one approach. Taking this approach has worked for years in the past and has been the only option…

The field of radio broadcast requires the cohesion of several different skill sets in order to be a success. KHEA Radio has used a traditional form of teaching, which means taking a one-on-one approach. Taking this approach has worked for years in the past and has been the only option for teaching. The down side to this method of teaching is that it requires one seasoned employee to stop their work and take the time to train a new employee. Because of the significant void in the area of instructional content for radio sound engineering, my co-worker and I had to troubleshoot this console and basically teach ourselves its functions. I saw the need for better instructional content on the Internet and in print based on my own experiences. The skills used to create the following instructional content were gained from course work at Arizona State University. The graduate department of Technical Communication makes every effort to equip students with varied skills that can be applied to different fields within the overall scheme of technical communication. This guide serves as a tool for radio broadcast novices to learn the basics of sound board operation.

ContributorsGarcia, Gerardo (Author) / D'Angelo, Barbara J. (Degree committee member) / Maid, Barry M. (Degree committee member) / Lauer, Claire (Degree committee member) / Arizona State University (Publisher)

Created2017-02-16

Usability Study for YourBrandPartner.com

Description

YourBrandPartner.com exists to provide content to those seeking specific advice and information on purchasing custom promotional items. For this investigation, I conducted a usability test with a select user group to identify user experience issues. The primary goal of this research was to conduct general usability testing through large group survey…

YourBrandPartner.com exists to provide content to those seeking specific advice and information on purchasing custom promotional items. For this investigation, I conducted a usability test with a select user group to identify user experience issues. The primary goal of this research was to conduct general usability testing through large group survey and a small in-person usability testing group. I designed surveys and tests to investigate if users experienced difficulties in finding the information they were looking for on the website. Based on the results of this study, I recommend reviewing the visual design of the website, increasing site speed, creating a better experience between the blog and e- commerce interactions, and creating an environment that is more accommodating of where the user is in the buying process. This full report includes expanded participant feedback, methodology behind the study, and full recommendations for improvement.

ContributorsWood, Amy (Author) / D'Angelo, Barbara J. (Degree committee member) / Batova, Tatiana (Degree committee member) / Maid, Barry M. (Degree committee member) / Arizona State University (Publisher)

Created2017-04-18

Understanding Parents' Health Information Needs When Their Child is Newly Diagnosed With Cancer

Description

The purpose of this applied project was to research and recommend to Phoenix Children’s Hospital (PCH) improvements to their website in order to provide parents whose child has been newly diagnosed with cancer the most clear and appropriate health information. I conducted a study in order to analyze and evaluate…

The purpose of this applied project was to research and recommend to Phoenix Children’s Hospital (PCH) improvements to their website in order to provide parents whose child has been newly diagnosed with cancer the most clear and appropriate health information. I conducted a study in order to analyze and evaluate the health information content currently provided to parents at PCH. This was done by through qualitative coding methods on both printed documents provided by The Emily Center Library, as well as interviews conducted with three Hematology/Oncology nurses at PCH. Additionally, I researched the current literature surrounding this topic in order to provide a background of information. Based on the results, I recommended that PCH offer parents a comprehensive cancer database in which all provided information would be searchable via their website. This database would also allow them to expand on their two topic focuses: home care and emotional support. Additionally, I recommended that parents are provided information on how to identify credible and non- credible sources on the Internet so that they can find information that is truly medically valuable when searching for information on their own. Lastly, I offered future recommendations that will require continued research so that PCH’s provided health information can continue to grow and improve.

ContributorsAudet, Tessa (Author) / Batova, Tatiana (Degree committee member) / D'Angelo, Barbara J. (Degree committee member) / Brumberger, Eva (Degree committee member) / Arizona State University (Publisher)

Created2017-04-17

Liberating Science: Accelerating the Innovation of Health Technology

Description

Translating research has been a goal of the Department of Health and Human Services since 1999. Through two years of iteration and interview with our community members, we have collected insights into the barriers to accomplishing this goal. Liberating Science is a think-tank of researchers and scientists who seek to…

Translating research has been a goal of the Department of Health and Human Services since 1999. Through two years of iteration and interview with our community members, we have collected insights into the barriers to accomplishing this goal. Liberating Science is a think-tank of researchers and scientists who seek to create a more transparent process to accelerate innovation starting with behavioral health research.

ContributorsRaghani, Pooja Sioux (Author) / Hekler, Eric (Thesis director) / Buman, Matthew (Committee member) / Pruthi, Virgilia Kaur (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor) / Biomedical Informatics Program (Contributor)

Created2014-05

Preliminary Metabolic Reconstruction of Two Methane Producing Microbes: Methanoregula boonei 6A8 and Methanosphaerula palustris E1-9c

Description

Methane (CH4) is very important in the environment as it is a greenhouse gas and important for the degradation of organic matter. During the last 200 years the atmospheric concentration of CH4 has tripled. Methanogens are methane-producing microbes from the Archaea domain that complete the final step in breaking down…

Methane (CH4) is very important in the environment as it is a greenhouse gas and important for the degradation of organic matter. During the last 200 years the atmospheric concentration of CH4 has tripled. Methanogens are methane-producing microbes from the Archaea domain that complete the final step in breaking down organic matter to generate methane through a process called methanogenesis. They contribute to about 74% of the CH4 present on the Earth's atmosphere, producing 1 billion tons of methane annually. The purpose of this work is to generate a preliminary metabolic reconstruction model of two methanogens: Methanoregula boonei 6A8 and Methanosphaerula palustris E1-9c. M. boonei and M. palustris are part of the Methanomicrobiales order and perform hydrogenotrophic methanogenesis, which means that they reduce CO2 to CH4 by using H2 as their major electron donor. Metabolic models are frameworks for understanding a cell as a system and they provide the means to assess the changes in gene regulation in response in various environmental and physiological constraints. The Pathway-Tools software v16 was used to generate these draft models. The models were manually curated using literature searches, the KEGG database and homology methods with the Methanosarcina acetivorans strain, the closest methanogen strain with a nearly complete metabolic reconstruction. These preliminary models attempt to complete the pathways required for amino acid biosynthesis, methanogenesis, and major cofactors related to methanogenesis. The M. boonei reconstruction currently includes 99 pathways and has 82% of its reactions completed, while the M. palustris reconstruction includes 102 pathways and has 89% of its reactions completed.

ContributorsMahendra, Divya (Author) / Cadillo-Quiroz, Hinsby (Thesis director) / Wang, Xuan (Committee member) / Stout, Valerie (Committee member) / Barrett, The Honors College (Contributor) / Computing and Informatics Program (Contributor) / School of Life Sciences (Contributor) / Biomedical Informatics Program (Contributor)

Created2014-05

Laberinto Journal Vol. 12 (2019)

ContributorsDe Armas, Frederick A., 1945- (Contributor) / Worden, Bill (Professor) (Contributor) / Marek, Margaret (Contributor) / Prendergast, Ryan (Contributor) / Gasior, Bonnie L., 1971- (Contributor) / Granja Ibarreche, Xabier (Contributor) / Gil-Osle, Juan Pablo (Contributor) / ACMRS Press (Creator) / Arizona State University (Contributor) / Arizona Center for Medieval and Renaissance Studies (Contributor)

Created2019

How the EnvZ/OmpR Two-component Regulatory System Affects fepA Gene Expression in Escherichia coli

Description

This study focused on the connection between the EnvZ/OmpR two-component regulatory system and the iron homeostasis system in Escherichia coli, specifically how a mutant form of EnvZ11/OmpR is able to reduce the expression of fepA::lacZ, a reporter gene fusion in E. coli. FepA is one of several outer membrane siderophore…

This study focused on the connection between the EnvZ/OmpR two-component regulatory system and the iron homeostasis system in Escherichia coli, specifically how a mutant form of EnvZ11/OmpR is able to reduce the expression of fepA::lacZ, a reporter gene fusion in E. coli. FepA is one of several outer membrane siderophore receptors that allow extracellular siderophores bound to iron to enter the cells to power various biological processes. Previous studies have shown that in E. coli cells that expressed a mutant allele of envZ, called envZ11, which led to altered expression of various iron genes including down regulation of fepA::lacZ. The wild type EnvZ/OmpR system is not considered to regulate iron genes, but because these envz11 strains had downregulated fepA::lacZ, this study was undertaken to understand the connection and mechanisms of this downregulation. A large number of Lac+ revertants were obtained from the B32-2483 strain (envz11 and fepA::lacZ) and 7 Lac+ revertants that had reversion mutations not directly correcting the envZ11 allele were further characterized. With P1 phage transduction genetic mapping that involved moving a kanamycin resistance marker linked to fepA::lacZ, two Lac+ revertants were found to have their reversion mutations in the fepA promoter region, while the other five revertants had their mutations mapping outside the fepA region. These two revertants underwent DNA sequencing and found to carry two different single base pair mutations in two different locations of the fepA promoter region. Each one is in the Fur repressor binding region, but one also may have affected the Shine-Dalgarno region involved in translation initiation. All 7 reveratants underwent beta-galactosidase assays to measure fepA::lacZ expression. The two revertants that had mutations in the fepA promoter region had significantly increased fepA activity, with the revertant with the Shine-Dalgarno mutation having the most elevated fepA expression. The other 5 revertants that did not map in the fepA region had fepA expression elevated to the same level as that found in the wild type EnvZ/OmpR background. The data suggest that the negative effect of envZ11 can be overcome by multiple mechanisms, including directly correcting the envZ11 allele or changing the fepA promoter region.

ContributorsKalinkin, Victor Arkady (Co-author) / Misra, Rajeev (Co-author, Thesis director) / Mason, Hugh (Committee member) / Foy, Joseph (Committee member) / Biomedical Informatics Program (Contributor) / School of Life Sciences (Contributor) / W. P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)

Created2016-05

Identification of structural mechanisms that modulate glycosaminoglycan affinity in various strains of decorin binding protein A

Description

Glycosaminoglycans (GAGs) are a class of complex biomolecules comprised of linear, sulfated polysaccharides whose presence on cell surfaces and in the extracellular matrix involve them in many physiological phenomena as well as in interactions with pathogenic microbes. Decorin binding protein A (DBPA), a Borrelia surface lipoprotein involved in the infectivity…

Glycosaminoglycans (GAGs) are a class of complex biomolecules comprised of linear, sulfated polysaccharides whose presence on cell surfaces and in the extracellular matrix involve them in many physiological phenomena as well as in interactions with pathogenic microbes. Decorin binding protein A (DBPA), a Borrelia surface lipoprotein involved in the infectivity of Lyme disease, is responsible for binding GAGs found on decorin, a small proteoglycan present in the extracellular matrix. Different DBPA strains have notable sequence heterogeneity that results in varying levels of GAG-binding affinity. In this dissertation, the structures and GAG-binding mechanisms for three strains of DBPA (B31 and N40 DBPAs from B. burgdorferi and PBr DBPA from B. garinii) are studied to determine why each strain has a different affinity for GAGs. These three strains have similar topologies consisting of five α-helices held together by a hydrophobic core as well as two long flexible segments: a linker between helices one and two and a C-terminal tail. This structural arrangement facilitates the formation of a basic pocket below the flexible linker which is the primary GAG-binding epitope. However, this GAG-binding site can be occluded by the flexible linker, which makes the linker a negative regulator of GAG-binding. ITC and NMR titrations provide KD values that show PBr DBPA binds GAGs with higher affinity than B31 and N40 DBPAs, while N40 binds with the lowest affinity of the three. Work in this thesis demonstrates that much of the discrepancies seen in GAG affinities of the three DBPAs can be explained by the amino acid composition and conformation of the linker. Mutagenesis studies show that B31 DBPA overcomes the pocket obstruction with the BXBB motif in its linker while PBr DBPA has a retracted linker that exposes the basic pocket as well as a secondary GAG-binding site. N40 DBPA, however, does not have any evolutionary modifications to its structure to enhance GAG binding which explains its lower affinity for GAGs. GMSA and ELISA assays, along with NMR PRE experiments, confirm that structural changes in the linker do affect GAG-binding and, as a result, the linker is responsible for regulating GAG affinity.

ContributorsMorgan, Ashli M (Author) / Wang, Xu (Thesis advisor) / Allen, James (Committee member) / Yarger, Jeffery (Committee member) / Arizona State University (Publisher)

Created2015

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