Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.

Long distance travel around the globe can potentially be revolutionized with the use of an intercontinental rocket that uses low earth orbit as its medium. This transport system can increase growth in many new businesses like tourism travel between the continents. This research evaluates the technical and non-technical possibilities of using a double-stage reusable rocket, where the second stage is also a reusable, rocket-powered passenger vehicle using a low earth orbit space journey with a stabilized re-entry method that ensures passenger comfortability. A potential network of spaceports spanning the globe is postulated within a range of 4,000 km to 8,000 km(2,160 nm to 4,320 nm) of each other, and each located within an hour by any other means of ground transport to population hubs greater than four million. This will help further connect the world as the journey from one major city to another would take at most an hour, and no point on the habited continents would be more than 4,000 km(2,160 nm) from a spaceport. It is assumed that the costs of an international first class flight ticket are in the thousands of dollars range showing how there is a potential market for this type of travel network. The reasoning and analysis, through a literature review, for an intercontinental rocket vehicle is presented along with the various aspects of the possibility of this kind of travel network coming to fruition in the near future.

Long distance travel around the globe can potentially be revolutionized with the use of an intercontinental rocket that uses low earth orbit as its medium. This transport system can increase growth in many new businesses like tourism travel between the continents. This research evaluates the technical and non-technical possibilities of using a double-stage reusable rocket, where the second stage is also a reusable, rocket-powered passenger vehicle using a low earth orbit space journey with a stabilized re-entry method that ensures passenger comfortability. A potential network of spaceports spanning the globe is postulated within a range of 4,000 km to 8,000 km(2,160 nm to 4,320 nm) of each other, and each located within an hour by any other means of ground transport to population hubs greater than four million. This will help further connect the world as the journey from one major city to another would take at most an hour, and no point on the habited continents would be more than 4,000 km(2,160 nm) from a spaceport. It is assumed that the costs of an international first class flight ticket are in the thousands of dollars range showing how there is a potential market for this type of travel network. The reasoning and analysis, through a literature review, for an intercontinental rocket vehicle is presented along with the various aspects of the possibility of this kind of travel network coming to fruition in the near future.

This paper outlines the design and testing of a z-scan spectrometer capable of measuring the third order refraction index of liquids. The spectrometer underwent multiple redesigns, with each explored in this paper with their benefits and drawbacks discussed. The first design was capable of measuring the third order refraction index for glass, and found a value of 8.43 +- 0.392 x 10^(-16) cm^2/W for the glass sample, with the literature stating glass has a refraction index between 1-100 x 10^(-16) cm^2/W. The second design was capable of measuring the third order refraction index of liquids, and found values of 1.23 $\pm$ 0.121 $\e{-16}$ and 9.43 +- 1.00 x 10^(-17) cm^2/W for water and ethanol respectively, with literature values of 2.7 x 10^(-16) and 5.0 x 10^(-17) cm^2/W respectively. The third design gave inconclusive results due to extreme variability in testing, and and the fourth design outlined has not been tested yet due to time constraints.

This research endeavor explores the 1964 reasoning of Irish physicist John Bell and how it pertains to the provoking Einstein-Podolsky-Rosen Paradox. It is necessary to establish the machinations of formalisms ranging from conservation laws to quantum mechanical principles. The notion that locality is unable to be reconciled with the quantum paradigm is upheld through analysis and the subsequent Aspect experiments in the years 1980-1982. No matter the complexity, any local hidden variable theory is incompatible with the formulation of standard quantum mechanics. A number of strikingly ambiguous and abstract concepts are addressed in this pursuit to deduce quantum's validity, including separability and reality. `Elements of reality' characteristic of unique spaces are defined using basis terminology and logic from EPR. The discussion draws directly from Bell's succinct 1964 Physics 1 paper as well as numerous other useful sources. The fundamental principle and insight gleaned is that quantum physics is indeed nonlocal; the door into its metaphysical and philosophical implications has long since been opened. Yet the nexus of information pertaining to Bell's inequality and EPR logic does nothing but assert the impeccable success of quantum physics' ability to describe nature.

Titanium has been and continues to be a popular metal across any form of manufacturing and production because of its extremely favorable properties. In important circumstances, it finds itself outclassing many metals by being lighter and less dense than comparably strong metals like steel. Relative to other metals it has a noteworthy corrosion resistance as it is stable when it oxidizes, and due to the inert nature of the metal, it is famously hypoallergenic and as a result used in a great deal of aviation and medical fields, including being used to produce replacement joints, with the notable limitation of the material being its cost of manufacturing. Among the variants of the metal and alloys used, Ti6Al4V alloy is famous for being the most reliable and popular combination for electron beam manufacturing(EBM) as a method of additive manufacturing. <br/>Developed by the Swedish Arcam, AB, EBM is one of the more recent methods of additive manufacturing, and is notable for its lack of waste by combining most of the material into the intended product due to its precision. This method, much like the titanium it is used to print in this case, is limited mostly by time and value of production. <br/>For this thesis, nine different simulations of a dogbone model were generated and analyzed in Ansys APDL using finite element analysis at various temperature and print conditions to create a theoretical model based on experimentally produced values.