Matching Items (324)
Description
The yeast project studies the growth of yeast Saccharomyces Cerevisiae (S. Cerevisiae) in high and low sulfate environments and analyzes the potential for genetically mutated plasmids to facilitate sulfate uptake in gene deficient yeast medias. The goal of the project was to transform the Sul1 and Sul2 transporters into the nutrient deficient yeast strain BY4743 and observe growth in conditions that would otherwise prohibit growth in order to create a model that can be used to study the effect of sulfate concentration on the transporters. The experimental results showed that expressing the sulfate transporters in the BY4743 strain provided the potential for the yeast to grow in nutrient-poor media. The growth potential model allows for further analysis on the sulfate transporters and will be used for research projects going forward.
ContributorsDickieson, Maxim Park (Author) / Nannenga, Brent (Thesis director) / Pena, Fred (Committee member) / Ira A. Fulton School of Engineering (Contributor) / Chemical Engineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Selective separation of environmentally relevant oxyanions is critical for water treatment and resource recovery. In this study, density functional theory (DFT) was used to evaluate the adsorption behavior of vanadate (H₂VO₄⁻), chromate (HCrO₄⁻), silicate (H₄SiO₄), and perchlorate (ClO₄⁻) on porphyrin derivatives. Modifications included functionalization, metalation, and a combination of both. The hypothesis posited that combining functionalization and metalation would enhance selectivity for toxic oxyanions (vanadate and chromate) over structurally similar, benign species. Binding energies revealed that functionalization alone did not improve selectivity, with silicate showing the strongest binding with an average of –0.780 eV due to extensive hydrogen bonding. Metalation significantly increased binding strength. V porphyrins in the 3+ state exhibited the following lowest binding energy values: –2.873 eV, –2.621 eV for chromate and vanadate, respectively. Selectivity, defined as a minimum of 0.1 eV difference in binding energy between target and competing adsorbates, was achieved in metallated systems, particularly for V 3+ porphyrins with chromate with a binding energy of 0.370 eV over silicate. The combination of functionalization and metalation offered minimal additional improvement over metallation.
These results highlight metalation, especially with high-valent vanadium and iron, as the dominant factor in enhancing selective oxyanion adsorption, whereas functionalization contributes little additional benefit. The findings support the rational design of metalloporphyrins for targeted contaminant removal applications.
ContributorsPark, Hannah (Author) / Muhich, Christopher (Thesis director) / D'arcy, Richard (Committee member) / Barrett, The Honors College (Contributor) / Chemical Engineering Program (Contributor)
Created2025-05
Description
Obesity has consistently presented a significant challenge, with excess body fat contributing to the development of numerous severe conditions such as diabetes, cardiovascular disease, cancer, and various musculoskeletal disorders. In this study, different methods are proposed to study substrate utilization (carbohydrates, proteins, and fats) in the human body and validate the biomarkers enabling to investigation of weight management and monitor metabolic health. The first technique to study was Indirect calorimetry, which assessed Resting Energy Expenditure (REE) and measured parameters like oxygen consumption (VO2) and carbon dioxide production (VCO2). A validation study was conducted to study the effectiveness of the medical device Breezing Med determining REE, VO2, and VCO2. The results were compared with correlation slopes and regression coefficients close to 1. Indirect Calorimetry can be used to determine carbohydrate and fat utilization but it requires additional correction for protein utilization. Protein utilization can be studied by analyzing urinary nitrogen. Therefore, a secondary technique was studied for identifying urea and ammonia concentration in human urine samples. Along this line two methods for detecting urea were explored, a colorimetric technique and it was validated against the Ion-Selective method. The results were then compared by correlation analysis of urine samples measured with both methods simultaneously curves. The equations for fat, carb, and protein oxidation, involving VO2, VCO2 consumption, and urinary nitrogen were implemented and validated, using the above-described methods in a human subject study with 16 subjects. The measurements included diverse diets (normal vs. high fat/protein) in normal energy balance and pre-/post interventions of exercise, fasting, and a high-fat meal. It can be concluded that the indirect calorimetry portable method in conjunction with urine urea methods are important to help the understanding of substrate utilization in human subjects, and therefore, excellent tools to contribute to the treatments and interventions of obesity and overweighted populations.
ContributorsPradhan, Ayushi (Author) / Forzani, Erica (Thesis advisor) / Lind, Mary Laura (Committee member) / Wang, Shaopeng (Committee member) / Arizona State University (Publisher)
Created2023
Description
The misuse of personal pronouns when referring to individuals has increased immensely, and as a result, misgendering has become commonplace. This creates uncomfortable social situations and can exacerbate already stressful situations such as meeting new people or traveling to new places. Taking information from my own experiences with these issues when abroad and combining with personal experiences from other individuals, the added stress due to misgendering can be addressed effectively. Data collection occurred in order to address awareness of situations, inclusivity of locations, and language when referring to people. A survey was used as a medium to gather the data. Questions asked related to the relationship between inclusivity and misgendering as well as unfamiliarity and travel. Information gathered in this study yielded two major takeaways: misgendering occurs similarly between individuals familiar and unfamiliar with the knowledge of gender-inclusivity and misgendering, and that further awareness of misgendering should be brought to the public. Further suggestions were created to better address the conclusions. The simplest action is to ask for an individual’s personal pronouns or gender identity when meeting them for the first time. This eliminates the chance of misgendering by unfamiliarity by making the other party familiar with the gender identity. The second suggestion is to use more words that do not address one gender in particular.
ContributorsGlaser, Sam (Author) / Briggs, Georgette (Thesis director) / Thomas, Kathy (Committee member) / Barrett, The Honors College (Contributor) / Chemical Engineering Program (Contributor)
Created2025-05