Nature journaling in school gardens is a unique way to engage students in the natural world, providing time to notice, wonder, and observe through writing and drawing while engaging in environmental and sustainability learning. With the number of school gardens increasing in the United States, educators can benefit from understanding what students experience while participating in garden-based learning activities so they can adapt their teaching to fit the educational needs of their participants. School garden studies typically focus on measuring academic and health outcomes and nature journaling studies typically focus on educator experiences with one classroom. We facilitated a nature journaling study with four elementary classrooms in a public, Title I school in the desert southwest to explore student experiences while nature journaling in their school garden. Our findings show that nature journaling provides opportunities for students to engage in sustainability literacy by helping them to develop a sense of place in their school garden, inspire environmental stewardship, and practice systems thinking right outside their classrooms.

For waste management in Asunción, Paraguay to improve, so too must the rate of public recycling participation. However, due to minimal public waste management infrastructure, it is up to individual citizens and the private sector to develop recycling solutions in the city. One social enterprise called Soluciones Ecológicas (SE) has deployed a system of drop-off recycling stations called ecopuntos, which allow residents to deposit their paper and cardboard, plastic, and aluminum. For SE to maximize the use of its ecopuntos, it must understand the perceived barriers to, and benefits of, their use. To identify these barriers and benefits, a doer on-doer survey based on the behavioral determinants outlined in the Designing for Behavior Change Framework was distributed among Asunción residents. Results showed that perceived self-efficacy, perceived social norms, and perceived positive consequences – as well as age – were influential in shaping ecopunto use. Other determinants such as perceived negative consequences, access, and universal motivators were significant predictors of gender and age. SE and other institutions looking to improve recycling can use these results to design effective behavior change interventions.

Often characterized by intense commoditization, heavy ecological footprint, and monopolistic governance mechanisms, the present-day industrialized food system has contributed to a growing distrust among citizens around the world. In response to this, local food initiatives promoting sustainable food and agricultural systems have formed. Little empirical research exists regarding how these local food initiatives think about their experience in relation a relevant conceptual framework. As such, this research entails the development of a conceptual framework based on Schlosberg and Cole’s (2015) sustainable materialist frame and literature regarding how local food initiatives may be characterized. This consists of sustainability values, collective action and political perspectives. Thirteen participants from two local food initiatives in Lüneburg, Germany were interviewed to provide in-depth insights into participant perceptions in relation to the three dimensions. Results indicate that participants exhibit strong values related to sustainability (e.g. knowledge of food origin, environmental concern, etc.), and appreciate the practical, collective work of the initiative. Additionally, a clash was found between initiative goals and participants’ perception of a lack of commitment and strong uniformity in the initiatives. Furthermore, many participants expressed political motivation and even perceived group actions as counter to the mainstream food system, although both initiatives did not identify as a politically motivated. This research sheds light on opportunities and barriers for initiative success and indicates the potential for the developed framework to serve as a lens for understanding other social initiatives aimed toward sustainability transformations.

Testing mediation models is critical for identifying potential variables that need to be targeted to effectively change one or more outcome variables. In addition, it is now common practice for clinicians to use multiple informant (MI) data in studies of statistical mediation. By coupling the use of MI data with statistical mediation analysis, clinical researchers can combine the benefits of both techniques. Integrating the information from MIs into a statistical mediation model creates various methodological and practical challenges. The authors review prior methodological approaches to MI mediation analysis in clinical research and propose a new latent variable approach that overcomes some limitations of prior approaches. An application of the new approach to mother, father, and child reports of impulsivity, frustration tolerance, and externalizing problems (N = 454) is presented. The results showed that frustration tolerance mediated the relationship between impulsivity and externalizing problems. The new approach allows for a more comprehensive and effective use of MI data when testing mediation models.

Cancer is sometimes depicted as a reversion to single cell behavior in cells adapted to live in a multicellular assembly. If this is the case, one would expect that mutation in cancer disrupts functional mechanisms that suppress cell-level traits detrimental to multicellularity. Such mechanisms should have evolved with or after the emergence of multicellularity. This leads to two related, but distinct hypotheses: 1) Somatic mutations in cancer will occur in genes that are younger than the emergence of multicellularity (1000 million years [MY]); and 2) genes that are frequently mutated in cancer and whose mutations are functionally important for the emergence of the cancer phenotype evolved within the past 1000 million years, and thus would exhibit an age distribution that is skewed to younger genes. In order to investigate these hypotheses we estimated the evolutionary ages of all human genes and then studied the probability of mutation and their biological function in relation to their age and genomic location for both normal germline and cancer contexts.

We observed that under a model of uniform random mutation across the genome, controlled for gene size, genes less than 500 MY were more frequently mutated in both cases. Paradoxically, causal genes, defined in the COSMIC Cancer Gene Census, were depleted in this age group. When we used functional enrichment analysis to explain this unexpected result we discovered that COSMIC genes with recessive disease phenotypes were enriched for DNA repair and cell cycle control. The non-mutated genes in these pathways are orthologous to those underlying stress-induced mutation in bacteria, which results in the clustering of single nucleotide variations. COSMIC genes were less common in regions where the probability of observing mutational clusters is high, although they are approximately 2-fold more likely to harbor mutational clusters compared to other human genes. Our results suggest this ancient mutational response to stress that evolved among prokaryotes was co-opted to maintain diversity in the germline and immune system, while the original phenotype is restored in cancer. Reversion to a stress-induced mutational response is a hallmark of cancer that allows for effectively searching “protected” genome space where genes causally implicated in cancer are located and underlies the high adaptive potential and concomitant therapeutic resistance that is characteristic of cancer.