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- Member of: ASU Electronic Theses and Dissertations
- Member of: The Center for Gender Equity in Science and Technology
DescriptionProvides results of a national study of digital technology use among African American teens and their parents.
ContributorsRideout, Victoria J. (Author) / Scott, Kimberley A., Ed.D. (Author) / Clark, Kevin A., Ph.D. (Author) / Arizona State University. Center for Gender Equity in Science and Technology (Contributor)
Created2016
DescriptionProvides an overview of strategies to network and communicate with those who influence policy creation.
ContributorsStigler, Monica L., Ph.D. (Author)
Created2020-12-02
DescriptionProvides detailed strategies to network and communicate with those who influence policy creation.
ContributorsStigler, Monica L., Ph.D. (Author)
Created2020-12-02
DescriptionProvides strategies for creating succinct presentations intended to persuade social policy decisionmakers.
ContributorsStigler, Monica L., Ph.D. (Author)
Created2020-08-01
DescriptionProvides results of a study that examined the effects of a girl-centered culturally responsive educational program on self-concept.
ContributorsTao, Chun, M.S. (Author) / Scott, Kimberly Ann, 1969- (Contributor) / Arizona State University. Center for Gender Equity in Science and Technology (Contributor)
Created2017 (year uncertain)
Description
Recording of educators and scholars discussing racial and gender inequalities in education for girls and women.
ContributorsWomen of Color in Computing Research Collaborative (Presenter)
Created2020-08-28
DescriptionProvides strategies to network and communicate with those who influence policy creation.
ContributorsStigler, Monica L., Ph.D. (Author)
Created2020-12-02
DescriptionRecording of educators and scholars discussing racial and gender inequalities for entrepreneurial women in the technical field.
ContributorsWomen of Color in Computing Research Collaborative (Presenter)
Created2020-09-21
Description
Informed by the CompuGirls program, the CompuPower: Technology and Leadership program curriculum uses culturally responsive education practices to foster student leadership and civic engagement through the exploration of technology. This program is a year-long, in-school, high school elective course that schools can offer to male and female students for Career and Technical Education (CTE) course credit.
ContributorsScott, Kimberly A. (Author) / Nkrumah, Tara (Author)
Created2020-06-05
Description
Glycosaminoglycans (GAGs) are a class of complex biomolecules comprised of linear, sulfated polysaccharides whose presence on cell surfaces and in the extracellular matrix involve them in many physiological phenomena as well as in interactions with pathogenic microbes. Decorin binding protein A (DBPA), a Borrelia surface lipoprotein involved in the infectivity of Lyme disease, is responsible for binding GAGs found on decorin, a small proteoglycan present in the extracellular matrix. Different DBPA strains have notable sequence heterogeneity that results in varying levels of GAG-binding affinity. In this dissertation, the structures and GAG-binding mechanisms for three strains of DBPA (B31 and N40 DBPAs from B. burgdorferi and PBr DBPA from B. garinii) are studied to determine why each strain has a different affinity for GAGs. These three strains have similar topologies consisting of five α-helices held together by a hydrophobic core as well as two long flexible segments: a linker between helices one and two and a C-terminal tail. This structural arrangement facilitates the formation of a basic pocket below the flexible linker which is the primary GAG-binding epitope. However, this GAG-binding site can be occluded by the flexible linker, which makes the linker a negative regulator of GAG-binding. ITC and NMR titrations provide KD values that show PBr DBPA binds GAGs with higher affinity than B31 and N40 DBPAs, while N40 binds with the lowest affinity of the three. Work in this thesis demonstrates that much of the discrepancies seen in GAG affinities of the three DBPAs can be explained by the amino acid composition and conformation of the linker. Mutagenesis studies show that B31 DBPA overcomes the pocket obstruction with the BXBB motif in its linker while PBr DBPA has a retracted linker that exposes the basic pocket as well as a secondary GAG-binding site. N40 DBPA, however, does not have any evolutionary modifications to its structure to enhance GAG binding which explains its lower affinity for GAGs. GMSA and ELISA assays, along with NMR PRE experiments, confirm that structural changes in the linker do affect GAG-binding and, as a result, the linker is responsible for regulating GAG affinity.
ContributorsMorgan, Ashli M (Author) / Wang, Xu (Thesis advisor) / Allen, James (Committee member) / Yarger, Jeffery (Committee member) / Arizona State University (Publisher)
Created2015