Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.

This paper describes how Arizona State University Library used creativity and novel approaches to collections design and implementation processes to select open stack print books for a newly renovated academic research library. Using results from a workshop focused on rethinking the future of print within educational learning and research environments, the Collections Services and Analysis unit within Arizona State University Library performed a series of experiments to better understand the purpose and use of print collections within 21st century library design. The authors describe the creative processes used in collections design and three types of selection approaches that invited engagement with open stacks. These three types were: small browsing collections co-curated with community members, a medium-sized print collection selected for student engagement, and a large research collection selected using a novel data analysis of four factors affecting the likelihood of potential use. Using more than one million volumes as the basis for selection, approximately 185,000 volumes were installed in the renovated library through a complex implementation across four library locations. The authors discuss the key role that creativity played in the approaches, methods, and results of these efforts and offer recommendations for collection management teams seeking to maximize their pursuit of community engagement with print collections within contemporary academic library spaces.