Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.

In his writings over the past decade, Brad Allenby has proposed (at least) 16 principles of sustainable engineering (see references) that are collectively known as the Earth Systems Engineering and Management (ESEM) principles. These principles have merit and applicability in many disciplines and domains of discourse, but are sometimes awkward to use due to the quantity of words required to accurately express their meaning. In light of this, it has become necessary to formulate a simplified list of “abbreviated tags” for ease of reference in conversation and concise writing. This list of tags also makes the principles immediately accessible to those who may want to pursue the more thorough definitions offered by Allenby. The following tags have been proposed for use when a concise phrasing is required. The citation provided after the tag is, in my opinion, the most complete expression of Allenby’s thought on this principle. It can be used when citing the principle in written assignments or publications.

Public transportation systems are often part of strategies to reduce urban environmental impacts from passenger transportation, yet comprehensive energy and environmental life-cycle measures, including upfront infrastructure effects and indirect and supply chain processes, are rarely considered. Using the new bus rapid transit and light rail lines in Los Angeles, near-term and long-term life-cycle impact assessments are developed, including consideration of reduced automobile travel. Energy consumption and emissions of greenhouse gases and criteria pollutants are assessed, as well the potential for smog and respiratory impacts.

Results show that life-cycle infrastructure, vehicle, and energy production components significantly increase the footprint of each mode (by 48–100% for energy and greenhouse gases, and up to 6200% for environmental impacts), and emerging technologies and renewable electricity standards will significantly reduce impacts. Life-cycle results are identified as either local (in Los Angeles) or remote, and show how the decision to build and operate a transit system in a city produces environmental impacts far outside of geopolitical boundaries. Ensuring shifts of between 20–30% of transit riders from automobiles will result in passenger transportation greenhouse gas reductions for the city, and the larger the shift, the quicker the payback, which should be considered for time-specific environmental goals.

This study seeks to examine how the introduction of residential solid oxide fuel cells (SOFC) will affect urban air quality. Both the life-cycle and operations emissions profiles of an SOFC are compared with the baseload electricity generating technologies that widespread adoption of SOFCs would replace – coal fired, natural gas combined cycle, and nuclear. The monetary impacts from use phase emissions are then assessed in five water-vulnerable cities in which SOFCs would likely be adopted in order to increase local resilience to electricity failures as a result of water shortages. The SOFC system under study is a 1 kWe system of planar design intended for residential CHP. The excess heat from the SOFC is used to heat domestic hot water. Analysis of the SOFC system life-cycle includes raw materials extraction and processing, component manufacturing, SOFC manufacturing, natural gas fuel processing and distribution, SOFC use, as well as energy used in these processes. Life-cycle analysis of the baseload power systems is bounded similarly. Emissions tracked for this study include SOx, NOx, VOCs, PM10, and PM2.5.